Embryo Culture System
Embryo culture confers different stages of embryogenesis at embryo transfer. The main stages at which embryo transfer is performed are cleavage stage or the blastocyst stage .Embryo culture until the blastocyst stage gives a significant increase in live birth rate per embryo transfer, but also gives a decreased number of embryos available for transfer and embryo cryopreservation, the cumulative clinical pregnancy rates is increased with cleavage stage transfer. Transfer day 2 instead of day 3 after fertilization will have no differences in rate. Embryos culture can be performed in an artificial culture medium or in an autologous endometrial co culture.With artificial culture medium, there either be same culture medium throughout the period, or a sequential system will be used, in this embryo is sequentially placed in different media. when culturing to blastocyst stage one medium is used for culture to day 3, and a second medium is used for culture. Sequential medium are equally effective for the culture of human embryos to the blastocyst stage. Artificial embryo culture media contain glucose, pyruvate, and energy-providing components, as the addition of amino acids, nucleotides, vitamins, and cholesterol improve the performance of embryonic growth and development. Methods to permit dynamic embryo culture with fluid flow and embryo movement is also available. A new method in the development uses the uterus as an incubator and naturally occurring intrauterine fluids as culture medium by encapsulating embryos in a permeable intrauterine vessel. IVF culture media was unable to identify a specific media that was superior in terms of pregnancy outcome. Low oxygen usage concentrations of 5% rather than about 20% in the atmosphere is shown to increase live birth rate to a relative probability of 1.24, without any evidence of increased risk for multiple pregnancies, miscarriages and congenital abnormalities.